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    r> Although generation of ROS is essential to p53-expression, cells un-dergoing p53-mediated apoptosis also generated ROS (Johnson et al., 1996). In fact, it has been reported that aberrant ROS-generation medi-ates p53-activation and apoptosis in HeLa cells. Interestingly, treatment of those cells with two antioxidants, N-acetylcysteine (NAC) and 4,5-di-hydroxy-1,3-benzenedisulfonic EPZ-6438 (tiron) significantly reduced the expression of p53 (Li, 1999).
    Taken together, these results indicate that the treatment induced both extrinsic and intrinsic apoptosis pathways and are inaccordance with previous study on KB human oral cancer cells treated with berber-ine (Kim et al., 2015).
    4. Conclusion
    Many studies showed that the use of medicinal plant extracts to con-trol or stop cancer progression should be an alternative to conventional treatment for this disease. In this work we demonstrated the antiprolif-erative effect of Algerian Berberis hispanica alkaloids extract on Hep-2 cancer cells and its ability to induce cell death through ROS-generation leading to morphological and molecular modifications, cell cycle arrest and induction of apoptosis. The results obtained open up prospects for a possible combination of this compound with existent anticancer drug already existing.
    Conflict of interest
    There are no conflicts of interests.
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